Fluorometric measurement of creatine kinase activity.

Observations on the fluorometric measurement of creatine kinase activity.

Twenty patients’ serums have been analyzed by both the Sax-Moore (1) and ConnAnido (2) creatine kinase methods. No significant difference in enzyme activity was found. In our hands, the Conn-Anido procedure, as originally described, gave variable results and high background fluorescence. The presence of high alkaline or acid phosphataseactivities in specimensanalyzed by the Sax-Moore procedure ...

A "reagentless" fluorometric method for creatine kinase activity in serum.

Measurement of serum creatine kinase (CK; EC 2.7.3.2) activity is of great value in the diagnosis of myocardial and skeletal muscle diseases (1-3). The greater specificity of CK over that of other enzymes, such as lactate dehydrogenase and aspartate aminotransferase, for the detection of muscular dystrophy and myocardial infarction has attracted much interest in CK determinations. In a prelimin...

In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility

Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase acti...

The Effect of Cigarette Smoking on Human Sperm Creatine Kinase Activity: As An ATP Buffering System in Sperm

In vitro Effect of Lead, Silver, Tin, Mercury, Indium and Bismuth on Human Sperm Creatine Kinase Activity: a Presumable Mechanism for Men Infertility

Background: The aim of the present study was to investigate the in vitro effects of mercury (Hg+2), lead (Pb+2), silver (Ag+2), tin (Sn+2), bismuth (Bi+3) and indium (In+3) ions on sperm creatine kinase. Methods: creatine kinase was isolated from human sperm homogenates after chromatography on a DEAE cellulose column. Results: At 60 µg ml-1 metal concentration, 70% of the creatine kinase activi...